Many inflammatory conditions start acutely via damage or infection, yet quickly spread to other organs. The process by which inflammation propagates through an organism is difficult to capture and understand if you study only a single organ. This team will develop a method to capture the exact state of single cells, including where the cells are positioned, for a whole animal. This method will clarify how an inflammatory signal — in this case sepsis — spreads, and what individual cells are responsible for sending and receiving information within tissues. Researchers will use the resulting data to model and simulate sepsis.